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Ammonium Test Strips 5.0-20.0 ppm Reflectoquant 1 PK *Req RQflex 10 #16950-1 HAZMAT fee incl

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Ammonium Test Strips 5.0-20.0 ppm Reflectoquant   1 PK *Req RQflex 10 #16950-1 HAZMAT fee incl MAIN
MSDS (Material Safety Data Sheet)

Reflectoquant® Ammomium Test, Catalog No. 16899/1

1. Method

NH4+ ions react with a chlorinating agent to form monochloramine. This in turn reacts with a phenol compound to form a blue indophenol derivative, the concentration of which is determined reflectometrically.

2. Measuring Range and Number of Determinations

Measuring range1) Number of Determinations
5.0-20.0mg/l NH4- 50

1) measurement of concentrations > 20mg/l NH4+ see section 6

3. Applications

Sample Material:
Soils and fertilizers

4. Influence of Foreign Substances

This was checked - in the case of interfering ions up to concentrations of 1,000 mg/l - in solutions containing 10mg/l NH4+. The concentrations of foreign substances given in the table lie below the limit at which the determination is interfered with:

Concentrations of foreign substances in mg/l or %
Al3+ 1,000

Ascorbate 100

BO33– 1000

Br 1,000

Ca2+ 1,000

Citrate 1,000

Cl 1,000

CN 1

CO32– 1,000

Cr3+ 100

CrO42– 100

Cu2+ 100
Fe2+ 10

Fe3+ 100

K+ 1,000

Mg2+ 1,000

Mn2+ 100

Ni2+ 1,000

NO2 50


Oxalate 1,000

PO43– 1,000

SO32– 100

Tartrate 1,000
EDTA 1,000

Anionic surfactants1) 1,000

Cationic surfactants2)1,000

Nonionic surfactants3) 100

H2O2 10

Na2SO4 5%

1) tested with Na-dodecyl sulfate

2) tested with N-cetylpyridinium chloride

3) tested with polyvinylpyrrolidone

5. Reagents and Auxiliaries

Please Note the Warnings on the packaging materials!
The analytical test strips are stable at least up to the date stated on the pack when stored in the closed tube at +2 to +8°C.

Package Contents:
Tube containing 50 analytical test strips
Bar code strip
1 bottle of reagent NH4-1
1 bottle of reagent NH42-
1 test vessel with stopper

Other Reagents:

EM Quant® Ammonium Test, Cat. No. 10024, measuring range 10-400mg/l NH4+
colorpHast® Universal Indicator Strips pH 0-14, Cat. No. 9590
Titristar®Sodium Hydroxide Solution 1mol/l, Cat. No. SX0607H
Titristar®Sulfuric Acid Solution 0.5mol/l, Cat. No. SX1243H
Ammonium Standard Solution ready to use, 1000mg/l, NH4+, Cat. No. 19812

6. Preparation

  • Check whether the ammonium content is within the measuring range
    Use the EM Quant® Ammonium Test as a guide. Samples containing more than 20.0mg/l NH4+ must be diluted with distilled water.
  • The pH must be within the range 4-13. Check with pH indicator strips. If pH is lower than 4, add sodium hydroxide solution dropwise; if greater than 13, add sulfuric acid dropwise.

7. Procedure

Observe the instructions for the RQflex Reflectometer.

The following applies to the Ammonium Test:

Measurement procedure A. Stored reaction time: 240 sec.

  • Rinse the test vessel several times with the pretreated sample solution (20-30°C). Then fill to the 5-ml mark.
  • Add 10 drops of reagent NH4 - 1 and swirl.
  • Add 1 level blue microspoon (in the cap of the NH4-2 bottle) of reagent NH4 -2 and swirl to dissolve.
  • Remove 1 analytical test strip and immediately close the tube.
  • Press START button of the reflectometer and - this is imperative - at the same time place the test strip in the measurement solution for 4 minutes.
  • 10 seconds before end of reaction time, remove the test strip from the solution and vigorously shake off excess liquid. Immediately insert the test strip all the way into the strip adapter with the reaction zones facing the display.
  • Read off the result from the display in mg/l NH4+. The result is automatically stored.


  • If the test strip is inserted into the adapter after the reaction time has expired, renewed depression of the START button may result in a false reading of the ammonium concentration.
  • At the end of each workday, cleanse the strip adapter thoroughly with water or ethanol.

8. Conversions

Units Required Units Given Conversion Factor
mmol/m3NH4+ or N mg/l NH4+ 55.4
mg/l N mg/l NH4+ 0.777
mg/l NH4+ mmol/m3 NH4+ or N 0.0180
mg/l NH4+ mg/l N 1.29

9. Method Control

To check analytical test strips, equipment, and procedure:

Dilute ammonium standard solution to 10mg/l NH4+ and analyze as described in section 7.

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