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Ascorbic Acid Test Strips 25-450 ppm Reflectoquant 50 PK *Requires RQflex 10 EMD# 16950-1

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Ascorbic Acid Test Strips 25-450 ppm Reflectoquant   50 PK *Requires RQflex 10 EMD# 16950-1 MAIN
MSDS (Material Safety Data Sheet)

Reflectoquant® Ascorbic Acid Test Strips, Catalog No. 16981/1

1. Method

Ascorbic acid reduces yellow molybdophosphoric acid to phosphomolybdenum blue, the concentration of which is determined reflectometrically.

2. Measuring Range and Number of Determinations

Measuring range1) Number of Determinations
25-450mg/l ascorbic acid 50

1) for measurement of concentrations > 450mg/l ascorbic acid, see section 6

3. Applications

Sample Material:

Food and food preserves (e.g. fruit and vegetable juices, beverages, beer, wine)

4. Influence of Foreign Substances

This was checked - in the case of interfering ions up to concentrations of 1,000mg/l - in solutions containing 200mg and 0mg/l ascorbic acid. The concentrations of foreign substances given in the table lie below the limit at which the determination is interfered with:

Concentrations of Foreign Substances inmg/l or %

Citrate 1,000 Oxalate 1,000
Fe2+ 10 SO32- 100
Fe3+ 10 Tartrate 1,000

5. Reagents and Auxiliaries

The analytical test strips are stable at least up to the date stated on the pack when stored in the closed tube at +15 to +25°C.

Package Contents:

Tube containing 50 analytical test strips
Bar code strip

Other Reagents:

EM Quant® Ascorbic Acid Test, Cat. No. 10023, measuring range 50-2,000mg/l ascorbic acid

Ascorbic acid GR, Cat. No. AX1775

6. Preparation

  • Check whether the ascorbic acid content is within the measuring range
  • Use the EM Quant® Ascorbic Acid Test as a guide. Samples containing more than 450mg/l ascorbic acid must be diluted with distilled water.

7. Procedure

  • Observe the instructions for the RQflex Reflectometer.

The following applies to the Ascorbic Acid Test:

Measurement procedure A. Stored reaction time: 15 sec.

  • Remove 1 analytical test strip and immediately reclose the tube.
  • Press START button of the Reflectometer and at the same time immerse both reaction zones of the test strip in the sample solution (5-30°C) for approx. 2 sec.
  • Shake off excess liquid from the test strip
  • As soon as the beeper sounds (5 sec. before end of reaction time), insert the test strip all the way into the strip adapter with the reaction zones facing the display.
  • Read off the result from the display. The result is automatically stored.


  • If the test strip is inserted into the adapter after the reaction time has expired, renewed depression of the START button may result in a false reading of the ascorbic acid concentration.
  • At the end of each workday, the strip adapter must be cleansed thoroughly with water or ethanol.

8. Method Control

To check analytical test strips, equipment and procedure:

Dissolve 0.1g ascorbic acid in distilled water, make up to 100ml, and mix. Ascorbic acid concentration 1,000mg/l.

Dilute this standard solution to 200mg/l ascorbic acid and analyze as described in section 7.

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