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Glucose Test Strips 1-100 ppm Reflectoquant 50 PK *Requires RQflex 10 EMD# 16950-1

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Glucose Test Strips 1-100 ppm Reflectoquant   50 PK *Requires RQflex 10 EMD# 16950-1 MAIN
MSDS (Material Safety Data Sheet)

Reflectoquant® Glucose Test Strips, Catalog No. 16720/1

1. Method

Under the catalytic effect of glucose oxidase, glucose is converted to gluconic acid lactone. The resultant hydrogen peroxide reacts with an organic redox indicator in the presence of peroxidase to form a blue-green dye, the concentration of which is determined reflectometrically.

2. Measuring Range and Number of Determinations

Measuring Range1) Number of Determinations
0-100 mg/l glucose 50

1) for measurement of concentrations >100mg/l glucose see section 6

3. Applications

Sample Material:

Fermentation solutions

4. Influence of Foreign Substances

This was checked - in the case of interfering ions up to concentrations of 1000 mg/l - in solutions containing 50mg/l glucose. The concentrations of foreign substances given in the table lie below the limit at which the determination is interfered with:

Concentrations of Foreign Substances in mg/l
Acetate 1000
Ascorbate 5
Ca2- 1000
Citrate 1000
Cl- 1000
CO32- 250
Lactate 1000
Malate 1000
NO3- 1000
Oxalate 1000
PO43- 1000
SO32- 1
Sorbate 1000
Ethanol 1000
Glycerol 1000

5. Reagents and Auxiliaries

The analytical test strips are stable at least up to the date stated on the pack when stored in the closed tube at +2 to +8°C.

Package Contents:

Tube containing 50 analytical test strips

Bar code strip

Other Reagents:

colorpHast® pH-indicator strips non-bleeding, Universal Indicator pH 0-14, Cat. No. 9590
Titristar® Sodium hydroxide solution 1mol/l, Cat. No. SX0607.
Hydrochloric acid 1mol/l, Cat. No. HX0603D
D(+)-Glucose anhydrous, Cat. No. DX0145

6. Preparation

Check whether the glucose content is within the measuring range. Samples containing more than 100mg/l glucose must be diluted with distilled water.

The pH must be within the range 2-10. Check with Universal Indicator Strips. If pH is lower than 2, add sodium hydroxide solution dropwise; if greater than 10, add hydrochloric acid dropwise.

7. Procedure

Observe the instructions for the RQflex Reflectometer.

The following applies to the glucose test:

Measurement procedure A. Stored reaction time: 60 sec.

  • Remove 1 analytical test strip and immediately reclose the tube.
  • Press START button of the reflectometer and - this is imperative - at the same time place the test strip for 15 sec in the solution to be tested (15-30°C) so that both reaction zones becomes completely wetted.
  • Shake off excess liquid from the test strip
  • As soon as the beeper sounds (5 sec. before end of reaction time), insert the test strip all the way into the strip adapter with the reaction zones facing the display.
  • Read off the result from the display in mg/l glucose. The result is automatically stored.


  • If the test strip is inserted into the adapter after the reaction time has expired, renewed depression of the START button may produce a false result.
  • At the end of each workday, cleanse the strip adapter thoroughly with water or ethanol.

8. Method Control

To check analytical test strips, equipment and procedure:

Dissolve 0.1g of anhydrous D(+)-glucose in distilled water, make up to 100ml, and mix.
Glucose concentration 1000mg/l
Leave this standard solution to stand for 2 hours, then dilute to 50mg/l glucose and analyze as described in section 7.

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