REFLECTOQUANT® Ammonium Test Strips, 20-180 ppm, 16977
Additional Production Information
Method: Reflectometric
Color Reaction: NH+4 ions react with Nessler's Reagent to form a yellow-brown compound, the concentration of which is determined reflectometrically.
Measuring Range: 20-180 ppm
Determinations: 50
Storage Conditions: +15 to +25°C
Shelf Life: 36 months (from day of production)
MSDS Material Safety Data Sheet PDF
Reflectoquant® Ammonium Test, Catalog No. 16977/1
1. Method
NH4+ ions react with Nessler's reagent to form a yellow-brown compound, the concentratin of which is determined reflectometrically.
2. Measuring Range and Number of Determinations
Measuring Range1) Number of Determinations
20 - 180 mg/l NH4+
15.5 - 140 mg/l N
1.110 - 9.970 mmol/m3 NH4+ or N 50
1) for conversion factors see section 8
for measurement of concentrations > 180 mg/l NH4+ see section 6
3. Applications
Sample Material:
Wastewater
Soils and fertilizers
4. Influence of Foreign Substances
This was checked - in the case of interfering ions up to concentrations of 1,000 mg/l - in solutions containing 100 mg and 0 mg//l NH4+. The concentrations of foreign substances given in the table lie below the limit at which the determination is interfered with:
Concentrations of Foreign Substances in mg/l or %
Al3+ 1,000 Fe2+ 10 EDTA 1,000
Ascorbate 1,000 Fe3+ 1,000 Anionic Surfactants1) 1,000
BO33- 1,000 K+ 1,000 Cationic Surfactants2) 100
Ca2+ 100 Mg2+ 1,000 Nonionic Surfactants3) 100
Citrate 1,000 Mn2+ 10 H2O2 1,000
Cl- 1,000 Ni2+ 100 Na2SO4 10%
CN- 10 NO2- 1,000
CO32- 1,000 NO3- 1,000
Cr3+ 100 Oxalate 1,000
CrO42- 1,000 PO43- 1,000
Cu2+ 1,000 S2O32- 1,000
Tartrate 1,000
1)tested with Na-dodecyl sulfate
2)tested with N-cetylpyridinium chloride
3)tested with polyvinylpyrrolidone
5. Reagents and Auxiliaries
Please Note the Warnings on Labels!
The analytic test strips are stable at least up to the date stated on the pack when stored in the closed tube at +15 to +25°C.
Package Contents:
Tube containing 50 analytical test strips
Bar code strip
2 bottle of reagent NH4 –1
1 test vessel
Other Reagents:
EM Quant® Ammonium Test, Cat. No. 10024, measuring range 10-400 mg/l NH4+
Ammonium standard solution ready for use, 1,000 mg/l Cat No. 19812
6. Preparation
Check whether the ammonium content is within the measuring range
Use the EM Quant® Ammonium Test as a guide. Samples containing more than 180 mg/l NH4+ must be diluted with distilled water.
7. Procedure
· Observe the instructions for the RQflex Reflectometer.
The following applies to the Ammonium Test:
Measurement procedure A. Stored reaction time: 15 sec.
Rinse the test vessel several times with the pretreated sample solution (15-25°C). Then fill to the 5-ml mark.
Add 10 drops of reagent NH4+ – 1 and swirl.
Remove 1 analytical test strip and immediately reclose the tube.
Press START button of the reflectometer and at the same time immerse both reaction zones of the test strip in the measurement solution for approx. 2 sec.
Vigorously shake off excess liquid from the test strip
As soon as the beeper sounds (5 sec. before end of reaction time), insert the test strip all the way into the strip adapter with the reaction zones facing the display.
Read off the result from the display in mg/l NH4+. The result is automatically stored.
Note:
At the end of each workday, cleanse the strip adapter thoroughly with water or ethanol
8. Conversions
units required = units given x conversion factor
mmol/m3 NH4+or N
mg/l N
mg/l NH4+
mg/l NH4+ mg/l NH4+
mg/l NH4+
mmol/m3 NH4+ or N
mg/l N 55.4
0.777
0.0180
1.29
9. Method Control
To check analytical test strips, equipment and procedure:
Dilute ammonium standard solution to 100 mg/l NH4+ and analyze as described in section 7.
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